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Analysis of protein aggregates by size exclusion chromatography (SEC)

Evaluation by SEC is effective for protein quality control!

Quality control of proteins, including biopharmaceuticals, is important, particularly regarding aggregates such as dimers and oligomers, as well as degradation products (low molecular weight species), which have gained attention in recent years due to their relationship with immunogenicity. Size exclusion chromatography (SEC) is widely used as a simple method for analyzing these. Here, we present examples of analyses conducted on commercially available protein mixtures, IgG antibodies, and IgG antibodies that were aggregated through pH stress testing. *For more details, please refer to the PDF document or feel free to contact us.

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